Document Type : Original Article
Authors
1
Department of Histology, Damietta Faculty of Medicine, Al-Azhar University, Egypt
2
Department of Physiology, Faculty of Medicine, Al-Azhar University, Egypt
3
Department of Physiology, Damietta Faculty of Medicine, Al-Azhar University, Egypt
4
Department of Anatomy and Embryology, Faculty of Medicine, Al-Azhar University, Egypt
5
Department of Internal Medicine, Faculty of Medicine for Girls, Al-Azhar University, Egypt
6
Department of Pharmacology, Faculty of Medicine, Helwan University, Egypt
7
Department of Forensic Medicine and Clinical Toxicology, Damietta Faculty of Medicine, Al-Azhar University, Egypt
8
Department of Anatomy and Embryology, Damietta Faculty of Medicine, Al-Azhar University, Egypt
Abstract
Background: Bisphenol A [BPA] is widely used in industrial processes. It persists in environment and exerts a harmful action on human health, through endocrine-disruption. Many natural compounds are suggested to have a preventive effect on BPA-induced human health hazards.
Aim of the work: This study aimed to evaluate the effect of Silymarin/Curcumin in ameliorating the hepatotoxic effect induced in albino rats following oral administration of BPA.
Patients and Methods: Fourty adult male albino rats were included and randomly categorized into four equal groups. The control group and BPA-exposed [50 mg/kg] group. Groups 3 and 4 for rats received oral BPA [50 mg/kg] plus [100 mg/ kg] of curcumin or silymarin. Serum liver enzymes, pro-inflammatory cytokines [TNF-α & IL-1,6,8] and the oxidant/antioxidant profile were assessed in the serum. SOD, GPX and CAT levels, MDA and H2O2 were assessed. Histopathological changes of the liver, immunohistochemical detection of caspase 3 and area density of collagen fibres were also assessed.
Results: Bisphenol-A administration for 30 days was associated with a significant increase in the number of pro-inflammatory markers as interleukin 1,6,8 and tumor necrosis factor‑α [TNF-α]. It also, stimulates oxidative stress with structural changes, apoptosis and fibrosis in hepatic cells. Treatment with curcumin or silymarin for 30 days lead to a reduction of pro‑inflammatory mediators and significant amelioration of structural changes.
Conclusion: Silymarin/Curcumin had an ameliorative of harmful effects of BPA on the liver cells through potent anti-inflammatory, antiapoptotic and antioxidant effects.
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