Effect of Vitamin D Supplementation with or without Metformin on Diabetes Mellitus in Adult Male Albino Rats

Document Type : Original Article

Authors

1 Department of Medical Physiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.

2 Department of Medical Physiology, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt.

Abstract

Background: Diabetes mellitus is characterized by hyperglycaemia and a variety of consequences. Vitamin D was an essential element in reducing the possibility of diabetes. Metformin has powerful antioxidant abilities in addition to its potential to treat diabetes.
Aim of the work: This study aimed to look into the role of vitamin D supplementation with or without metformin in diabetes mellitus in adult male albino rats.
Patients and methods: The current study examined the effect of vitamin D supplementation with or without metformin on diabetes mellitus in adult male albino rats of the local strain, weighing between 130 and 150 g. Rats were split into six equal groups at Random. The first group negative control group, where rats received regular diet every day for 4 weeks. The second was the positive control, where it received vehicle. Group III was the alloxan induced diabetic group, while group IV was the diabetic group with vitamin supplementation, and the fifth group was the diabetic plus metformin-treated group. The last group was the diabetic group with vitamin D and metformin treatment. Rats were given ether anaesthesia at the conclusion of the trial and blood samples were taken before the following metrics were assessed: blood glucose, vitamin D, alanine aminotransferase [ALT], aspartate aminotransferase [AST], alkaline phosphatase [ALP], homeostatic model assessment for insulin resistance [HOMA-IR] and malondialdehyde [MDA].
Results: Blood glucose, ALT, AST, alkaline phosphatase, HOMA-IR, and MDA were significantly reduced when vitamin D, metformin or both were supplemented. The prior metrics improved more when metformin and vitamin D were administered together.
Conclusion: Vitamin-D is promising for treatment of diabetes-related issues [e.g., hyperglycaemia and insulin resistance]. Also, metformin has been shown to improve blood glucose and when addition vitamin D with metformin it showed better results that led to near normal levels in diabetes.

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